HIF1A Antibody / HIF-1 alpha

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NSJ Bioreagents
Product Code: NSJ-RQ5614
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5614-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the HIF-1 alpha antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) U-87 MG, 2) U-2 OS, 3) PC-3, 4) A549 and 5) HepG2 lysate with HIF-1 alpha antibody. Routinely observed molecular weight: 100~120 kDa.
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Western blot testing of human 1) U-87 MG, 2) U-2 OS, 3) PC-3, 4) A549 and 5) HepG2 lysate with HIF-1 alpha antibody. Routinely observed molecular weight: 100~120 kDa.

Documents

Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the HIF-1 alpha antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
HIF-1a (Hypoxia-inducible factor 1 alpha or HIF1A) is a transcription factor that mediates cellular and systemic homeostatic responses to reduced O2 availability in mammals, including angiogenesis, erythropoiesis and glycolysis. This gene was mapped to 14q21-q24. HIF-1a transactivate genes required for energy metabolism and tissue perfusion and is necessary for embryonic development and tumor explant growth. HIF-1alpha is over expressed during carcinogenesis, myocardial infarction and wound healing. It is crucial for the cellular response to hypoxia and is frequently over expressed in human cancers, resulting in the activation of genes essential for cell survival. HIF-1a regulates the survival and function in the inflammatory microenvironment directly. It is a transcription factor that plays a pivotal role in cellular adaptation to changes in oxygen availability.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids H197-R718) was used as the immunogen for the HIF-1 alpha antibody.
Limitation:
This HIF-1 alpha antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
Q16665