Claudin 2 Antibody / CLDN2

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NSJ Bioreagents
Product Code: NSJ-RQ6463
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6463-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the Claudin 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human Caco-2 cells with Claudin 2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Flow cytometry testing of human A549 cells with Claudin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Claudin 2 antibody.
3 / 3
Western blot testing of human Caco-2 cell lysate with Claudin 2 antibody. Predicted molecular weight ~25 kDa.
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Immunofluorescent staining of FFPE human Caco-2 cells with Claudin 2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A549 cells with Claudin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Claudin 2 antibody.
Western blot testing of human Caco-2 cell lysate with Claudin 2 antibody. Predicted molecular weight ~25 kDa.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Claudin 2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Claudin-2 is a protein that in humans is encoded by the CLDN2 gene. By genomic sequence analysis, this gene is mapped to chromosome Xq22.3-q23. This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. By expression in a human intestinal epithelial cell line, it was determined that the intestine-specific homeodomain proteins CDX1 and CDX2 activated a reporter plasmid driven by the CLDN2 promoter.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An E. coli-derived human protein (amino acids Q63-V230) was used as the immunogen for the Claudin 2 antibody.
Limitation:
This Claudin 2 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P57739