Deoxyhypusine synthase Antibody / DHPS / DHS

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NSJ Bioreagents
Product Code: NSJ-RQ6469
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6469-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Deoxyhypusine synthase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 5
IHC staining of FFPE human gallbladder adenocarcinoma tissue with Deoxyhypusine synthase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human lung cancer tissue with Deoxyhypusine synthase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
Immunofluorescent staining of FFPE human MCF7 cells with Deoxyhypusine synthase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 5
Wester blot testing of human 1) HeLa, 2) MCF7, 3) Jurkat, 4) HEK293, 5) SH-SY5Y, 6) ThP-1, 7) PC-3, 8) U937 and 9) rat testis cell lysate with Deoxyhypusine synthase antibody. Expected molecular weight: 36-40 kDa.
5 / 5
Flow cytometry testing of human K562 cells with Deoxyhypusine synthase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Deoxyhypusine synthase antibody.
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IHC staining of FFPE human gallbladder adenocarcinoma tissue with Deoxyhypusine synthase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with Deoxyhypusine synthase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human MCF7 cells with Deoxyhypusine synthase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Wester blot testing of human 1) HeLa, 2) MCF7, 3) Jurkat, 4) HEK293, 5) SH-SY5Y, 6) ThP-1, 7) PC-3, 8) U937 and 9) rat testis cell lysate with Deoxyhypusine synthase antibody. Expected molecular weight: 36-40 kDa.
Flow cytometry testing of human K562 cells with Deoxyhypusine synthase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Deoxyhypusine synthase antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Deoxyhypusine synthase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Deoxyhypusine synthase is an enzyme that in humans is encoded by the DHPS gene. This gene encodes a protein that is required for the formation of hypusine, a unique amino acid formed by the posttranslational modification of only one protein, eukaryotic translation initiation factor 5A. The encoded protein catalyzes the first step in hypusine formation by transferring the butylamine moiety of spermidine to a specific lysine residue of the eukaryotic translation initiation factor 5A precursor, forming an intermediate deoxyhypusine residue. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An E. coli-derived human protein (amino acids F54-K358) was used as the immunogen for the Deoxyhypusine synthase antibody.
Limitation:
This Deoxyhypusine synthase antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P49366