EHD1 Antibody / EH domain-containing protein 1 / PAST1

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NSJ Bioreagents
Product Code: NSJ-RQ6475
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6475-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the PAST1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human SiHa cells with PAST1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human T47-D, 2) human Caco-2, 3) human A549, 4) human MCF7, 5) monkey COS-7, 6) mouse testis, 7) mouse kidney, 8) mouse lung and 9) mouse ovary lysate with PAST1 antibody. Expected molecular weight ~61 kDa.
3 / 3
Flow cytometry testing of human SiHa cells with PAST1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PAST1 antibody.
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Immunofluorescent staining of FFPE human SiHa cells with PAST1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human T47-D, 2) human Caco-2, 3) human A549, 4) human MCF7, 5) monkey COS-7, 6) mouse testis, 7) mouse kidney, 8) mouse lung and 9) mouse ovary lysate with PAST1 antibody. Expected molecular weight ~61 kDa.
Flow cytometry testing of human SiHa cells with PAST1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PAST1 antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the PAST1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
EH domain-containing protein 1, also known as testilin or PAST homolog 1 (PAST1), is a protein that in humans is encoded by the EHD1 gene, belonging to the EHD protein family. It is mapped to 11q13.1. This gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The protein encoded by this gene is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids HELPADLPPHLVPPSKRRHE from the human protein were used as the immunogen for the PAST1 antibody.
Limitation:
This PAST1 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Monkey
Uniprot #:
Q9H4M9