EHMT2 Antibody / G9A

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NSJ Bioreagents
Product Code: NSJ-RQ6477
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6477-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the EHMT2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 6
IHC staining of FFPE human liver cancer tissue with EHMT2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human rectal cancer tissue with EHMT2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE mouse ovary tissue with EHMT2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
Immunofluorescent staining of FFPE human A549 cells with EHMT2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
5 / 6
Western blot testing of human 1) PC-3, 2) HEK293, 3) U-87 MG, 4) A549, 5) U-2 OS and 6) rat testis lysate with EHMT2 antibody. Expected molecular weight: 160-180 kDa.
6 / 6
Flow cytometry testing of human HL60 cells with EHMT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= EHMT2 antibody.
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IHC staining of FFPE human liver cancer tissue with EHMT2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer tissue with EHMT2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse ovary tissue with EHMT2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A549 cells with EHMT2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) PC-3, 2) HEK293, 3) U-87 MG, 4) A549, 5) U-2 OS and 6) rat testis lysate with EHMT2 antibody. Expected molecular weight: 160-180 kDa.
Flow cytometry testing of human HL60 cells with EHMT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= EHMT2 antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the EHMT2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Euchromatic histone-lysine N-methyltransferase 2 (EHMT2), also known as G9a, is a histone methyltransferase enzyme that in humans is encoded by the EHMT2 gene. This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An E. coli-derived human protein (amino acids Q580-H1195) was used as the immunogen for the EHMT2 antibody.
Limitation:
This EHMT2 antibody is available for research use only.
Localization:
Nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q96KQ7