Beta Enolase Antibody / ENO3 / Enolase 3

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NSJ Bioreagents
Product Code: NSJ-RQ6479
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6479-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Beta Enolase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human Caco-2 cells with Beta Enolase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta Enolase antibody.
2 / 2
Western blot testing of 1) human HeLa, 2) human A549, 3) human U937, 4) human Caco-2, 5) rat skeletal muscle, 6) rat heart, 7) mouse skeletal muscle and 8) mouse heart lysate with Beta Enolase antibody. Predicted molecular weight ~47 kDa.
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Flow cytometry testing of human Caco-2 cells with Beta Enolase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta Enolase antibody.
Western blot testing of 1) human HeLa, 2) human A549, 3) human U937, 4) human Caco-2, 5) rat skeletal muscle, 6) rat heart, 7) mouse skeletal muscle and 8) mouse heart lysate with Beta Enolase antibody. Predicted molecular weight ~47 kDa.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Beta Enolase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Enolase 3 (ENO3), also known as beta-enolase and muscle specific enolase, is an enzyme that in humans is encoded by the ENO3 gene. This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An E. coli-derived human protein (amino acids K60-K103) was used as the immunogen for the Beta Enolase antibody.
Limitation:
This Beta Enolase antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P13929