HDAC2 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6487
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6487-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the HDAC2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 11
IHC staining of FFPE human ovarian cancer tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 11
IHC staining of FFPE human gastric cancer tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 11
IHC staining of FFPE human cervical cancer tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 11
IHC staining of FFPE human breast cancer tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 11
IHC staining of FFPE human endometrial cancer tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 11
IHC staining of FFPE human gallbladder adenocarcinoma tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 11
IHC staining of FFPE human lung cancer tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 11
IHC staining of FFPE mouse testis tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 11
Immunofluorescent staining of FFPE human Caco-2 cells with HDAC2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
10 / 11
Western blot testing of 1) human K562, 2) human ThP-1, 3) human HL60, 4) human Caco-2, 5) human HepG2, 6) rat brain, 7) rat lung, 8) rat pancreas, 9) mouse brain and 10) mouse lung lysate with HDAC2 antibody. Predicted molecular weight: 55-60 kDa.
11 / 11
Flow cytometry testing of human HL60 cells with HDAC2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HDAC2 antibody.
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IHC staining of FFPE human ovarian cancer tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gastric cancer tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human cervical cancer tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human endometrial cancer tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gallbladder adenocarcinoma tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse testis tissue with HDAC2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human Caco-2 cells with HDAC2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human K562, 2) human ThP-1, 3) human HL60, 4) human Caco-2, 5) human HepG2, 6) rat brain, 7) rat lung, 8) rat pancreas, 9) mouse brain and 10) mouse lung lysate with HDAC2 antibody. Predicted molecular weight: 55-60 kDa.
Flow cytometry testing of human HL60 cells with HDAC2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HDAC2 antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the HDAC2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Histone deacetylase 2 is an enzyme that in humans is encoded by the HDAC2 gene. This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones(H2A, H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Betz et al.(1998) performed PCR using HDAC2-specific primers to screen a somatic cell hybrid mapping panel. They mapped the HDAC2 gene to human chromosome 6q21, a region of the genome altered in some cancers, including retinoblastoma.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An E. coli-derived human protein (amino acids E387-P488) was used as the immunogen for the HDAC2 antibody.
Limitation:
This HDAC2 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q92769