KCNQ2 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6494
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6494-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the KCNQ2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human renal clear cell carcinoma tissue with KCNQ2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human lung cancer tissue with KCNQ2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human rectal cancer tissue with KCNQ2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human breast cancer tissue with KCNQ2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Western blot testing of 1) human SH-SY5Y, 2) human HEK293, 3) rat brain, 4) rat brain, 5) rat C6, 6) mouse brain and 7) mouse brain lysate with KCNQ2 antibody. Predicted molecular weight ~96 kDa.
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Flow cytometry testing of human 293T cells with KCNQ2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KCNQ2 antibody.
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Flow cytometry testing of rat C6 cells with KCNQ2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KCNQ2 antibody.
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IHC staining of FFPE human renal clear cell carcinoma tissue with KCNQ2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with KCNQ2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer tissue with KCNQ2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with KCNQ2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human SH-SY5Y, 2) human HEK293, 3) rat brain, 4) rat brain, 5) rat C6, 6) mouse brain and 7) mouse brain lysate with KCNQ2 antibody. Predicted molecular weight ~96 kDa.
Flow cytometry testing of human 293T cells with KCNQ2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KCNQ2 antibody.
Flow cytometry testing of rat C6 cells with KCNQ2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KCNQ2 antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the KCNQ2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
KCNQ2, also called kv7.2, is a potassium channel protein coded for by the gene KCNQ2. It is mapped to 20q13.33. The KCNQ2 gene encodes a voltage-gated potassium channel that is expressed in the brain. Expression of human KCNQ2 in Xenopus laevis oocytes led to potassium-selective currents that activated slowly with depolarization. Defects in this gene are a cause of benign familial neonatal convulsions type 1 (BFNC), also known as epilepsy, benign neonatal type 1(EBN1). At least five transcript variants encoding five different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An E. coli-derived human protein (amino acids M371-D408) was used as the immunogen for the KCNQ2 antibody.
Limitation:
This KCNQ2 antibody is available for research use only.
Localization:
Cell membrane, cytoplasm
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O43526