MAPK12 Antibody / p38 gamma

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NSJ Bioreagents
Product Code: NSJ-RQ6495
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6495-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the p38 gamma antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human HeLa cells with p38 gamma antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HeLa, 2) human HEK293, 3) monkey COS-7, 4) human K562, 5) rat skeletal muscle and 6) mouse skeletal muscle lysate with p38 gamma antibody. Expected molecular weight: 38-42 kDa.
3 / 3
Flow cytometry testing of human U-2 OS cells with p38 gamma antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= p38 gamma antibody.
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Immunofluorescent staining of FFPE human HeLa cells with p38 gamma antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human HEK293, 3) monkey COS-7, 4) human K562, 5) rat skeletal muscle and 6) mouse skeletal muscle lysate with p38 gamma antibody. Expected molecular weight: 38-42 kDa.
Flow cytometry testing of human U-2 OS cells with p38 gamma antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= p38 gamma antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the p38 gamma antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Mitogen-activated protein kinase 12 (MAP kinase 12), also known as p38 gamma, extracellular signal-regulated kinase 6 (ERK6) or stress-activated protein kinase 3 (SAPK3), is an enzyme that in humans is encoded by the MAPK12 gene. Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. The protein encoded by this gene functions as a signal transducer during differentiation of myoblasts to myotubes.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An E. coli-derived human protein (amino acids S229-L367) was used as the immunogen for the p38 gamma antibody.
Limitation:
This p38 gamma antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P53778