Neurogranin Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6501
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6501-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the NRGN antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human K562 cells with NRGN antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NRGN antibody.
2 / 2
Western blot testing of 1) human PC-3, 2) rat brain and 3) mouse brain lysate with NRGN antibody. Predicted molecular weight: ~8 kDa but routinely observed at ~17 kDa.
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Flow cytometry testing of human K562 cells with NRGN antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NRGN antibody.
Western blot testing of 1) human PC-3, 2) rat brain and 3) mouse brain lysate with NRGN antibody. Predicted molecular weight: ~8 kDa but routinely observed at ~17 kDa.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the NRGN antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Neurogranin is a calmodulin-binding protein expressed primarily in the brain, particularly in dendritic spines, and participating in the protein kinase C signaling pathway. Neurogranin (NRGN) is the human homolog of the neuron-specific rat RC3/neurogranin gene. This gene encodes a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. The NRGN gene contains four exons and three introns. The exons 1 and 2 encode the protein and exons 3 and 4 contain untranslated sequences. It is suggested that the NRGN is a direct target for thyroid hormone in human brain, and that control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An E. coli-derived human protein (amino acids K11-K54) was used as the immunogen for the NRGN antibody.
Limitation:
This NRGN antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q92686