OAT Antibody / Ornithine aminotransferase

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NSJ Bioreagents
Product Code: NSJ-RQ6502
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6502-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Ornithine Aminotransferase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 5
IHC staining of FFPE human lung cancer tissue with Ornithine Aminotransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human rectal cancer tissue with Ornithine Aminotransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human testicular cancer tissue with Ornithine Aminotransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of human 1) K562, 2) A431, 3) HEK293, 4) Caco-2, 5) U-87 MG, 6) HepG2, 7) U-2 OS, 8) rat kidney, 9) rat testis, 10) mouse brain and 11) mouse kidney lysate with Ornithine Aminotransferase antibody. Predicted molecular weight ~49 kDa.
5 / 5
Flow cytometry testing of human A549 cells with Ornithine Aminotransferase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ornithine Aminotransferase antibody.
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IHC staining of FFPE human lung cancer tissue with Ornithine Aminotransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer tissue with Ornithine Aminotransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human testicular cancer tissue with Ornithine Aminotransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) K562, 2) A431, 3) HEK293, 4) Caco-2, 5) U-87 MG, 6) HepG2, 7) U-2 OS, 8) rat kidney, 9) rat testis, 10) mouse brain and 11) mouse kidney lysate with Ornithine Aminotransferase antibody. Predicted molecular weight ~49 kDa.
Flow cytometry testing of human A549 cells with Ornithine Aminotransferase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ornithine Aminotransferase antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Ornithine Aminotransferase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Ornithine aminotransferase (OAT) is an enzyme which is encoded in human by the OAT gene located on chromosome 10. This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An E. coli-derived human protein (amino acids A214-F439) was used as the immunogen for the Ornithine Aminotransferase antibody.
Limitation:
This Ornithine Aminotransferase antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P04181