PPP4R1 Antibody / PP4R1

NSJ Bioreagents
Product Code: NSJ-RQ6508
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6508-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the PPP4R1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 2
Flow cytometry testing of human A431 cells with PPP4R1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPP4R1 antibody.
2 / 2
Western blot testing of 1) monkey COS-7, 2) human K562, 3) rat brain, 4) rat stomach, 5) mouse brain and 6) mouse stomach lysate with PPP4R1 antibody. Expected molecular weight: 107-125 kDa.

Flow cytometry testing of human A431 cells with PPP4R1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPP4R1 antibody.
Western blot testing of 1) monkey COS-7, 2) human K562, 3) rat brain, 4) rat stomach, 5) mouse brain and 6) mouse stomach lysate with PPP4R1 antibody. Expected molecular weight: 107-125 kDa.

Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the PPP4R1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Serine/threonine-protein phosphatase 4 regulatory subunit 1 is an enzyme that in humans is encoded by the PPP4R1 gene. This gene encodes one of several alternate regulatory subunits of serine/threonine protein phosphatase 4 (PP4). The protein features multiple HEAT repeats. This protein forms a complex with PP4RC. This complex may have a distinct role from other PP4 complexes, including regulation of HDAC3 (Zhang et al., PMID: 15805470). There is also a transcribed pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids HQEAVEQTIMALQMDRDSDVKYFASIH from the human protein were used as the immunogen for the PPP4R1 antibody.
Limitation:
This PPP4R1 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat, Monkey
Uniprot #:
Q8TF05