Arylsulfatase C Antibody / Steryl-sulfatase / ARSC

NSJ Bioreagents
Product Code: NSJ-RQ6523
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6523-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Steryl-sulfatase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
IHC staining of FFPE human placental tissue with Steryl-sulfatase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Flow cytometry testing of human U-87 MG cells with Steryl-sulfatase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Steryl-sulfatase antibody.
3 / 3
Western blot testing of human placental tissue lysate with Steryl-sulfatase antibody. Predicted molecular weight ~65 kDa.

IHC staining of FFPE human placental tissue with Steryl-sulfatase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human U-87 MG cells with Steryl-sulfatase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Steryl-sulfatase antibody.
Western blot testing of human placental tissue lysate with Steryl-sulfatase antibody. Predicted molecular weight ~65 kDa.

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Steryl-sulfatase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Steroid sulfatase (STS), or steryl-sulfatase, formerly known as arylsulfatase C, is a sulfatase enzyme involved in the metabolism of steroids. It is encoded by the STS gene. This gene encodes a multi-pass membrane protein that is localized to the endoplasmic reticulum. It belongs to the sulfatase family and hydrolyzes several 3-beta-hydroxysteroid sulfates, which serve as metabolic precursors for estrogens, androgens, and cholesterol. Mutations in this gene are associated with X-linked ichthyosis (XLI). Alternatively spliced transcript variants resulting from the use of different promoters have been described for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An E. coli-derived human protein (amino acids K297-Q547) was used as the immunogen for the Steryl-sulfatase antibody.
Limitation:
This Steryl-sulfatase antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P08842