DDX6 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6541
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6541-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 8G6
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the DDX6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Flow cytometry testing of human HeLa cells with DDX6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DDX6 antibody.
2 / 3
Western blot testing of human 1) HeLa, 2) HepG2, 3) SW620, 4) Raji, 5) ThP-1, 6) Jurkat, 7) rat PC-12 and 8) mouse NIH 3T3 cell lysate with DDX6 antibody. Predicted molecular weight: ~53 kDa.
3 / 3
Immunofluorescent staining of FFPE human MCF7 cells with DDX6 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Flow cytometry testing of human HeLa cells with DDX6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DDX6 antibody.
Western blot testing of human 1) HeLa, 2) HepG2, 3) SW620, 4) Raji, 5) ThP-1, 6) Jurkat, 7) rat PC-12 and 8) mouse NIH 3T3 cell lysate with DDX6 antibody. Predicted molecular weight: ~53 kDa.
Immunofluorescent staining of FFPE human MCF7 cells with DDX6 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the DDX6 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
DDX6 (DEAD/H BOX 6), also known as HLR2 or p54, is an enzyme that in humans is encoded by the DDX6 gene. DDX6 belongs to the DEAD box family of putative RNA helicases that contain a characteristic asp-glu-ala-asp (DEAD) box motif (Seto et al., 1995). Tunnacliffe et al.(1993) assigned the DDX6 gene more precisely using a panel of sequence tagged sites (STSs) representing 30 markers previously assigned to 11q23. Using mass spectroscopy, Fenger-Gron et al.(2005) found that RCK, EDC3(YJDC), and HEDLS (RCD8) co-immunopurified with DCP1A and DCP2 from HEK293 cell lysates. Overexpression of DCP2, RCK, or EDC3 in HeLa cells reduced the association of endogenous DCP1A and XRN1 with cytoplasmic P bodies.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An E. coli-derived human protein (amino acids Q56-P483) was used as the immunogen for the DDX6 antibody.
Limitation:
This DDX6 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P26196