CLIC1 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6545
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6545-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the CLIC1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human A431 cells with CLIC1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CLIC1 antibody.
2 / 2
Western blot testing of 1) human HL60, 2) human HeLa, 3) monkey COS-7, 4) human ThP-1, 5) human Caco-2, 6) human U937 and 7) rat kidney lysate with CLIC1 antibody. Expected molecular weight: 27-30 kDa.
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Flow cytometry testing of human A431 cells with CLIC1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CLIC1 antibody.
Western blot testing of 1) human HL60, 2) human HeLa, 3) monkey COS-7, 4) human ThP-1, 5) human Caco-2, 6) human U937 and 7) rat kidney lysate with CLIC1 antibody. Expected molecular weight: 27-30 kDa.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CLIC1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Chloride intracellular channel protein 1 is a protein that in humans is encoded by the CLIC1 gene. Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An E. coli-derived human protein (amino acids D47-K241) was used as the immunogen for the CLIC1 antibody.
Limitation:
This CLIC1 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Rat, Monkey
Uniprot #:
O00299