Dicer Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6547
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6547-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the Dicer antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human MCF7 cells with Dicer antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human K562, 2) human HepG2, 3) human RT4 and 4) mouse NIH 3T3 cell lysate with Dicer antibody. Expected molecular weight: 219-250 kDa.
3 / 3
Flow cytometry testing of human Caco-2 cells with Dicer antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Dicer antibody.
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Immunofluorescent staining of FFPE human MCF7 cells with Dicer antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human K562, 2) human HepG2, 3) human RT4 and 4) mouse NIH 3T3 cell lysate with Dicer antibody. Expected molecular weight: 219-250 kDa.
Flow cytometry testing of human Caco-2 cells with Dicer antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Dicer antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Dicer antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Dicer (DICER1), also known as endoribonuclease Dicer or helicase with RNase motif, is an enzyme that in humans is encoded by the DICER1 gene. It is mapped to 14q32.13. The DICER1 gene, a member of the ribonuclease III (RNase III) family, is involved in the generation of microRNAs (miRNAs), which modulate gene expression at the post-transcriptional level. DICER1 possesses an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus DICER, also known as helicase-MOI, is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. In addition, DICER1 is required for formation of the RNA induced silencing complex (RISC). It also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An E. coli-derived human protein (amino acids Y1546-S1922) was used as the immunogen for the Dicer antibody.
Limitation:
This Dicer antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q9UPY3