Dnmt3l Antibody

NSJ Bioreagents
Product Code: NSJ-RQ6549
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6549-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Dnmt3l antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 2
Flow cytometry testing of mouse ANA-1 cells with Dnmt3l antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Dnmt3l antibody.
2 / 2
Western blot testing of 1) rat kidney and 2) mouse kidney tissue lysate with Dnmt3l antibody. Predicted molecular weight ~49 kDa.

Flow cytometry testing of mouse ANA-1 cells with Dnmt3l antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Dnmt3l antibody.
Western blot testing of 1) rat kidney and 2) mouse kidney tissue lysate with Dnmt3l antibody. Predicted molecular weight ~49 kDa.

Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Dnmt3l antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
DNA (cytosine-5)-methyltransferase 3-like is an enzyme that in humans is encoded by the DNMT3L gene. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein that is a catalytically inactive regulatory factor of DNA methyltransferases. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An E. coli-derived mouse protein (amino acids A43-L421) was used as the immunogen for the Dnmt3l antibody.
Limitation:
This Dnmt3l antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
Q9CWR8