Hjurp Antibody / Fleg1

NSJ Bioreagents
Product Code: NSJ-RQ6561
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6561-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Hjurp antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 2
Flow cytometry testing of mouse ANA-1 cells with Hjurp antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Hjurp antibody.
2 / 2
Western blot testing of 1) mouse stomach, 2) mouse liver, 3) mouse kidney, 4) mouse RAW264.7, 5) rat stomach, 6) rat liver and 7) rat kidney lysate with Hjurp antibody. Expected molecular weight: 74-90 kDa.

Flow cytometry testing of mouse ANA-1 cells with Hjurp antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Hjurp antibody.
Western blot testing of 1) mouse stomach, 2) mouse liver, 3) mouse kidney, 4) mouse RAW264.7, 5) rat stomach, 6) rat liver and 7) rat kidney lysate with Hjurp antibody. Expected molecular weight: 74-90 kDa.

Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Hjurp antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Holliday junction recognition protein is a protein in humans that is encoded by the HJURP gene. HJURP (holliday junction recognition protein) is a protein coding gene. Diseases associated with HJURP include fibrillary astrocytoma. Among its related pathways are DNA damage and chromosome maintenance. Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds holliday junctions.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An E. coli-derived mouse protein (amino acids Q13-T574) was used as the immunogen for the Hjurp antibody.
Limitation:
This Hjurp antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
Q6PG16