HTR1B Antibody / 5HT1B

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NSJ Bioreagents
Product Code: NSJ-RQ6562
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6562-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the 5HT1B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 6
IHC staining of FFPE human rectal cancer tissue with 5HT1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human pancreatic cancer tissue with 5HT1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE mouse brain tissue with 5HT1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE rat brain tissue with 5HT1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
Western blot testing of human 1) A431, 2) HeLa, 3) rat brain, 4) rat C6, 5) mouse brain and 6) mouse Neuro-2a cell lysate with 5HT1B antibody. Predicted molecular weight ~44 kDa.
6 / 6
Flow cytometry testing of human A549 cells with 5HT1B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= 5HT1B antibody.
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IHC staining of FFPE human rectal cancer tissue with 5HT1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human pancreatic cancer tissue with 5HT1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with 5HT1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with 5HT1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) A431, 2) HeLa, 3) rat brain, 4) rat C6, 5) mouse brain and 6) mouse Neuro-2a cell lysate with 5HT1B antibody. Predicted molecular weight ~44 kDa.
Flow cytometry testing of human A549 cells with 5HT1B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= 5HT1B antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the 5HT1B antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
5-hydroxytryptamine receptor 1B also known as the 5-HT1B receptor is a protein that in humans is encoded by the HTR1B gene. The protein encoded by this intronless gene is a G-protein coupled receptor for serotonin (5-hydroxytryptamine). Ligand binding activates second messengers that inhibit the activity of adenylate cyclase and manage the release of serotonin, dopamine, and acetylcholine in the brain. The encoded protein may be involved in several neuropsychiatric disorders and therefore is often a target of antidepressant and other psychotherapeutic drugs.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An E. coli-derived human protein (amino acids A35-S390) was used as the immunogen for the 5HT1B antibody.
Limitation:
This 5HT1B antibody is available for research use only.
Localization:
Cytoplasm, cell membrane
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P28222