Cd25 Antibody / IL2ra

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NSJ Bioreagents
Product Code: NSJ-RQ6563
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6563-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the IL-2 receptor alpha antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 4
IHC staining of FFPE mouse spleen tissue with IL-2 receptor alpha antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE rat small intestine tissue with IL-2 receptor alpha antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of 1) mouse ANA-1, 2) mouse NIH 3T3, 3) mouse RAW264.7, 4) mouse HEPA-1, 5) rat C6, 6) rat NRK and 7) rat RH35 cell lysate with IL-2 receptor alpha antibody. Expected molecular weight: 25~45 kDa depending on level of glycosylation.
4 / 4
Flow cytometry testing of mouse HEPA1-6 cells with IL-2 receptor alpha antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IL-2 receptor alpha antibody.
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IHC staining of FFPE mouse spleen tissue with IL-2 receptor alpha antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat small intestine tissue with IL-2 receptor alpha antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) mouse ANA-1, 2) mouse NIH 3T3, 3) mouse RAW264.7, 4) mouse HEPA-1, 5) rat C6, 6) rat NRK and 7) rat RH35 cell lysate with IL-2 receptor alpha antibody. Expected molecular weight: 25~45 kDa depending on level of glycosylation.
Flow cytometry testing of mouse HEPA1-6 cells with IL-2 receptor alpha antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IL-2 receptor alpha antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the IL-2 receptor alpha antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
IL2RA (Interleukin 2 receptor, alpha), also called IL2R, IL2R, Alpha Chain or CD25, is a protein that in humans is encoded by the IL2RA gene. The IL2RA gene is mapped on 10p15.1. IL2RA is composed of strand-swapped sushi-like domains, unlike the classical cytokine receptor fold. As a result of this domain swap, IL2RA uses a composite surface to dock into a groove on IL2 that also serves as a binding site for antagonist drugs. Homodimeric alpha chains(IL2RA) result in low-affinity receptor, while homodimeric beta(IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Infection by the protozoan Trypanosoma cruzi causes Chagas disease, characterized by a reduction in the amount of IL2RA expressed on the surface of immune cells. This leads to chronic immune suppression, becoming increasingly severe over the course of many years and ultimately resulting in death if left untreated.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids PWKHEDSKRIYHFVE from the mouse protein were used as the immunogen for the IL-2 receptor alpha antibody.
Limitation:
This IL-2 receptor alpha antibody is available for research use only.
Localization:
Cell surface, Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
P01590