ILK Antibody / Integrin linked kinase

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NSJ Bioreagents
Product Code: NSJ-RQ6564
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6564-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Integrin Linked Kinase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 9
Immunofluorescent staining of FFPE human A431 cells with Integrin Linked Kinase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 9
IHC staining of FFPE human renal clear cell carcinoma tissue with Integrin Linked Kinase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 9
IHC staining of FFPE human lung cancer tissue with Integrin Linked Kinase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 9
IHC staining of FFPE mouse colon tissue with Integrin Linked Kinase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 9
IHC staining of FFPE rat kidney tissue with Integrin Linked Kinase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 9
Western blot testing of 1) human HeLa, 2) human HEK293, 3) rat kidney, 4) rat spleen, 5) rat PC-12 and 6) mouse spleen lysate with Integrin Linked Kinase antibody. Expected molecular weight: 51-59 kDa.
7 / 9
Flow cytometry testing of human Raji cells with Integrin Linked Kinase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Integrin Linked Kinase antibody.
8 / 9
Flow cytometry testing of mouse RAW264.7 cells with Integrin Linked Kinase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Integrin Linked Kinase antibody.
9 / 9
Flow cytometry testing of rat C6 cells with Integrin Linked Kinase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Integrin Linked Kinase antibody.
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Immunofluorescent staining of FFPE human A431 cells with Integrin Linked Kinase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human renal clear cell carcinoma tissue with Integrin Linked Kinase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with Integrin Linked Kinase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse colon tissue with Integrin Linked Kinase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat kidney tissue with Integrin Linked Kinase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human HEK293, 3) rat kidney, 4) rat spleen, 5) rat PC-12 and 6) mouse spleen lysate with Integrin Linked Kinase antibody. Expected molecular weight: 51-59 kDa.
Flow cytometry testing of human Raji cells with Integrin Linked Kinase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Integrin Linked Kinase antibody.
Flow cytometry testing of mouse RAW264.7 cells with Integrin Linked Kinase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Integrin Linked Kinase antibody.
Flow cytometry testing of rat C6 cells with Integrin Linked Kinase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Integrin Linked Kinase antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Integrin Linked Kinase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
ILK, also known as Integrin-linked kinase, is a serine-threonine protein kinase. Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK) interacts with the cytoplasmic domain of beta-1 integrin. This gene was initially described to encode a serine/ threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. Recent results showed that ILK contains 5 ankyrin-like repeats, and that the C-terminal kinase domain is actually a pseudo-kinase with adaptor function.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An E. coli-derived human protein (amino acids M1-K452) was used as the immunogen for the Integrin Linked Kinase antibody.
Limitation:
This Integrin Linked Kinase antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q13418