KLC1 Antibody / Kinesin light chain 1

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NSJ Bioreagents
Product Code: NSJ-RQ6566
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6566-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the KLC1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human PC-3 cells with KLC1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Western blot testing of human 1) HeLa, 2) U-87 MG, 3) SH-SY5Y, 4) COLO-320, 5) HepG2, 6) PC-3 and 7) HACAT cell lysate with KLC1 antibody. Predicted molecular weight: 63-72 kDa (multiple isoforms).
3 / 4
Western blot testing of 1) rat brain, 2) rat skeletal muscle, 3) mouse brain and 4) mouse lung tissue lysate with KLC1 antibody. Predicted molecular weight: 63-72 kDa (multiple isoforms).
4 / 4
Flow cytometry testing of human Caco-2 cells with KLC1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KLC1 antibody.
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Immunofluorescent staining of FFPE human PC-3 cells with KLC1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) U-87 MG, 3) SH-SY5Y, 4) COLO-320, 5) HepG2, 6) PC-3 and 7) HACAT cell lysate with KLC1 antibody. Predicted molecular weight: 63-72 kDa (multiple isoforms).
Western blot testing of 1) rat brain, 2) rat skeletal muscle, 3) mouse brain and 4) mouse lung tissue lysate with KLC1 antibody. Predicted molecular weight: 63-72 kDa (multiple isoforms).
Flow cytometry testing of human Caco-2 cells with KLC1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KLC1 antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the KLC1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Kinesin light chain 1 is a protein that in humans is encoded by the KLC1 gene. Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains, and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity, while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain, and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles, mitochondria, and the Golgi complex. Thus, kinesin light chains function as adapter molecules and not motors per se. Although previously named kinesin 2, this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however, the full-length nature and/or biological validity of most of these variants have not been determined.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An E. coli-derived human protein (amino acids M1-D537) was used as the immunogen for the KLC1 antibody.
Limitation:
This KLC1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q07866