FAM129B Antibody / MINERVA / NIBAN2

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NSJ Bioreagents
Product Code: NSJ-RQ6569
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6569-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the FAM129B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 4
IHC staining of FFPE human lung cancer tissue with FAM129B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human placental tissue with FAM129B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of 1) human HeLa, 2) human RT4, 3) rat stomach, 4) rat C6, 5) mouse stomach and 6) mouse NIH 3T3 cell lysate with FAM129B antibody. Predicted molecular weight ~84 kDa.
4 / 4
Flow cytometry testing of human HeLa cells with FAM129B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FAM129B antibody.
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IHC staining of FFPE human lung cancer tissue with FAM129B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with FAM129B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human RT4, 3) rat stomach, 4) rat C6, 5) mouse stomach and 6) mouse NIH 3T3 cell lysate with FAM129B antibody. Predicted molecular weight ~84 kDa.
Flow cytometry testing of human HeLa cells with FAM129B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FAM129B antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the FAM129B antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
FAM129B/Niban-like protein 1 (family with sequence similarity 129, member B) belongs to a poorly characterized family of Niban proteins that also includes FAM129A/Niban and FAM129C/Niban-like protein 2. FAM129A/Niban is implicated in the ER stress response and is upregulated at the protein level in thyroid carcinoma. FAM129C/Niban-like protein 2 is preferentially expressed in B-cells and is one of five biomarkers upregulated in whole blood from patients with colorectal carcinoma. FAM129B is broadly expressed and has been shown to be a downstream target of B-Raf in melanoma cells. Though FAM129B does not appear to regulate cell growth and division, phosphorylation of FAM129B by B-Raf is essential for the invasive potential of melanoma and non-melanoma cell lines. Deletion of FAM129B in melanoma cells significantly impairs B-Raf/MEK/Erk-dependent invasion into the extracellular matrix.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An E. coli-derived human protein (amino acids K23-K561) was used as the immunogen for the FAM129B antibody.
Limitation:
This FAM129B antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
Q96TA1