ZC3H12A (CT) Antibody

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ProSci
Product Code: PSI-9547
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9547-0.02mg0.02mg£150.00
Quantity:
PSI-9547-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
ZC3H12A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1. WB Validation in Human Lung </strong><br> Loading: 15 μg of lysate per lane. Antibodies: ZC3H12A 9547, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 2 μg/mL Lane 2: 4 μg/mL
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<strong>Figure 2 Western Blot Validation in Mouse Tissues </strong><br> Loading: 15 μg of lysates per lane. Antibodies: ZC3H12A 9547, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 3. Western Blot Validation in Rat Tissues </strong><br> Loading: 15 μg of lysates per lane. Antibodies: ZC3H12A 9547, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 4. Immunofluorescence Validation of ZC3H12A in Human THP-1 Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed THP-1 cells labeling ZC3H12A with 9547 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 5. Immunohistochemistry Validation of ZC3H12A in Human Testis</strong><br> Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ZC3H12A antibody (9547) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 6. Immunohistochemistry Validation of ZC3H12A in Mouse Liver</strong><br> Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ZC3H12A antibody (9547) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin
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<strong>Figure 7. Immunohistochemistry Validation of ZC3H12A in Rat Brain</strong><br> Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ZC3H12A antibody (9547) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 1. WB Validation in Human Lung </strong><br> Loading: 15 μg of lysate per lane. Antibodies: ZC3H12A 9547, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 2 μg/mL Lane 2: 4 μg/mL
<strong>Figure 2 Western Blot Validation in Mouse Tissues </strong><br> Loading: 15 μg of lysates per lane. Antibodies: ZC3H12A 9547, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 3. Western Blot Validation in Rat Tissues </strong><br> Loading: 15 μg of lysates per lane. Antibodies: ZC3H12A 9547, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 4. Immunofluorescence Validation of ZC3H12A in Human THP-1 Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed THP-1 cells labeling ZC3H12A with 9547 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 5. Immunohistochemistry Validation of ZC3H12A in Human Testis</strong><br> Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ZC3H12A antibody (9547) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6. Immunohistochemistry Validation of ZC3H12A in Mouse Liver</strong><br> Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ZC3H12A antibody (9547) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin
<strong>Figure 7. Immunohistochemistry Validation of ZC3H12A in Rat Brain</strong><br> Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ZC3H12A antibody (9547) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Documents

Further Information

Additional Names:
ZC3H12A Antibody: Endoribonuclease ZC3H12A, Monocyte chemotactic protein-induced protein 1, MCP-induced protein 1, MCPIP-1, Zinc finger CCCH domain-containing protein 12A
Application Note:
WB: 2 μg mL; IF: 10 μg mL; IHC: 1 μg mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples; Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.
Background:
ZC3H12A Antibody: ZC3H12A is an MCP1 (CCL2; MIM 158105)-induced protein that acts as a transcriptional activator and causes cell death of cardiomyocytes, possibly via induction of genes associated with apoptosis. ZC3H12A inhibits the Wnt/beta-catenin signaling pathway activity and modulates epithelial-mesenchymal transition during clear cell renal cell carcinoma progression by targeting miRNAs. ZC3H12A ribonuclease can bind and cleave AURKA mRNA in MYCN-amplified neuroblastoma cells. ZC3H12A is a novel link between diabetogenic conditions and impaired insulin secretory capacity. ZC3H12A overexpression in human neuroblastoma cell lines causes cell-cycle arrest by G1/S checkpoint block.
Background References:
  • Gorka et al. Oncogene. 2021; 40(50):6720-6735
  • Nowak et al. RNA Biol. 2021; 18(1):144-156
  • Tyka et al. Biochim Biophys Acta Mol Basis Dis. 2021; 1867(10):166199
  • Boratyn et al. J Cell Biochem. 2020; 121(5-6);3406-3425
Buffer:
ZC3H12A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
Homology:
Predicted species reactivity based on immunogen sequence: Chimpanzee (100%); Monkey (100%); Pig (79%); Chicken (83%)
Immunogen:
Anti-ZC3H12A antibody (9547) was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus of human ZC3H12A.

The immunogen is located within the last 50 amino acids of ZC3H12A.
ISOFORMS:
Human ZC3H12A has one isoform (599aa, 65.7kD). Mouse has one isoform (596aa, 65.6kD). Rat has one isoform (596aa, 65.9kD). 9547 can detect ZC3H12A in human, mouse, and rat.
NCBI Gene ID #:
80149
NCBI Official Name:
zinc finger CCCH-type containing 12A
NCBI Official Symbol:
ZC3H12A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 66 kD
Observed: 66 kD
Protein Accession #:
Q5D1E7.2
Protein GI Number:
190485853
Purification:
ZC3H12A Antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
Swissprot #:
Q5D1E8
User NOte:
Optimal dilutions for each application to be determined by the researcher.