POLRMT (CT) Antibody

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ProSci
Product Code: PSI-9561
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9561-0.02mg0.02mg£150.00
Quantity:
PSI-9561-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
POLRMT antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 WB Validation in Human Cell Lines </strong><br> Loading: 15 μg of lysate. Antibodies: POLRMT, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 2 Immunofluorescence Validation of POLRMT in Daudi Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling POLRMT with 9561 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 3 Immunofluorescence Validation of POLRMT in Mouse Brain</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain labeling POLRMT with 9561 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 4 Immunofluorescence Validation of POLRMT in Rat Brain</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed rat brain labeling POLRMT with 9561 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 1 WB Validation in Human Cell Lines </strong><br> Loading: 15 μg of lysate. Antibodies: POLRMT, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 2 Immunofluorescence Validation of POLRMT in Daudi Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling POLRMT with 9561 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 3 Immunofluorescence Validation of POLRMT in Mouse Brain</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain labeling POLRMT with 9561 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 4 Immunofluorescence Validation of POLRMT in Rat Brain</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed rat brain labeling POLRMT with 9561 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Documents

Further Information

Additional Names:
DNA-directed RNA polymerase, mitochondrial, MtRPOL, POLRMT Isoforms
Application Note:
WB: 2 μg/mL; IF: 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
Background:
POLRMT Antibody: This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes. TFB2M and POLRMT are essential for mammalian mitochondrial DNA replication. POLRMT mutations impair mitochondrial transcription causing neurological disease. Knock-down of MRPL12 by RNA interference results in instability of POLRMT.
Background References:
  • Inatomi et al. Biochim Biophys Acta Mol Cell Res. 2022; 1869(1); 119167
  • Olahova et al. Nat Commun. 2021; 12(1):1135
  • Nouws et al. J Biol Chem 2016; 291(2):989-997
Buffer:
POLRMT Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
Homology:
Predicted species reactivity based on immunogen sequence: Cow (82%); Chimpanzee (100%) ; monkey (82%); Cat (90%)
Immunogen:
Anti-POLRMT antibody (9561) was raised against a peptide corresponding to 11 amino acids near the carboxyl terminus of human POLRMT.
The immunogen is located within the last 50 amino acids of POLRMT.
ISOFORMS:
Human POLRMT has 1 isoform (1230aa, 138.6kD). Mouse POLRMT has one isoform (1207aa, 136.7kD). Rat POLRMT has one isoform (1203aa, 135.8kD). 9561 can detect POLRMT in human, mouse and rat.
NCBI Gene ID #:
5442
NCBI Official Name:
RNA polymerase mitochondrial
NCBI Official Symbol:
POLRMT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 139kD
Observed: 139 kD
Protein Accession #:
O0411.2
Protein GI Number:
50403691
Purification:
POLRMT Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
O0411
User NOte:
Optimal dilutions for each application to be determined by the researcher.