FZD6 (IN) Antibody

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ProSci
Product Code: PSI-9551
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9551-0.02mg0.02mg£150.00
Quantity:
PSI-9551-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
FZD6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 WB Validation in Human, Mouse and Rat Cell Lines</strong><br> Loading: 15 μg of lysate. Antibodies: FZD6 9551, 2 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 2 Immunofluorescence Validation of FZD6 in Daudi Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling FZD6 with 9551 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 3 Immunofluorescence Validation of FZD6 in Mouse Lung</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung labeling FZD6 with 9551 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 4 Immunofluorescence Validation of FZD6 in Rat Lung</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung labeling FZD6 with 9551 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 1 WB Validation in Human, Mouse and Rat Cell Lines</strong><br> Loading: 15 μg of lysate. Antibodies: FZD6 9551, 2 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 2 Immunofluorescence Validation of FZD6 in Daudi Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling FZD6 with 9551 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 3 Immunofluorescence Validation of FZD6 in Mouse Lung</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung labeling FZD6 with 9551 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 4 Immunofluorescence Validation of FZD6 in Rat Lung</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung labeling FZD6 with 9551 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Documents

Further Information

Additional Names:
FZD6 Antibody: Frizzled-6, Fz-6, hFz6
Application Note:
WB: 2 μg/mL; IF: 10 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
Background:
FZD6 Antibody: FZD6 acts as a receptor for Wnt proteins. Frizzled receptors are coupled to the beta-catenin canonical signaling pathway. Together with FZD3, FZD6 is involved with neural tube closure and plays a role in the regulation of planar cell polarity, particularly in the orientation of asymmetric bundles of stereocilia on apical faces of a subset of sensory cells located in the inner ear.
Background References:
  • Wang et al. J. Neurosci. 206;26(8):2147-2156
Buffer:
FZD6 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
Homology:
Predicted species reactivity based on immunogen sequence: Chimpanzee(100%); Monkey (100%); Dog (100%)
Immunogen:
Anti-FZD6 antibody (9551) was raised against a peptide corresponding to 14 amino acids in the central domain of human FZD6.
The immunogen is located within 50-550 amino acids of FZD6.
ISOFORMS:
Human FZD6 has 2 isoforms, including isoform 1 (706aa, 79.3kD) and isoform 2 (674aa, 75.6kD). Mouse FZD6 has 1 isoform (709aa, 79kD). Rat FZD6 has one isoform (710aa, 79.1kD). 9551 can detect FZD6 in human, mouse and rat.
NCBI Gene ID #:
8323
NCBI Official Name:
frizzled class receptor 6
NCBI Official Symbol:
FZD6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 79kD
Observed: 95 kD (Post-translational modification: 2 N-linked glycosylations)
Protein Accession #:
O60353
Protein GI Number:
215273986
Purification:
FZD6 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cell Cycle, Signal Transduction, Stem Cell, Growth Factors
Swissprot #:
O60353
User NOte:
Optimal dilutions for each application to be determined by the researcher.