IFI16 (CT) Antibody

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ProSci
Product Code: PSI-9641
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9641-0.02mg0.02mg£150.00
Quantity:
PSI-9641-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
IFI16 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 WB Validation in Human and Rat Cell Lines </strong><br> Loading: 15 μg of lysate per lane. Antibodies: IFI16 9641, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 2 Western Blot Validation in Mouse Tissues </strong><br> Loading: 15 μg of lysates per lane. Antibodies: IFI16 9641, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 3 Western Blot Validation in Rat Testis</strong><br> Loading: 15 μg of lysate per lane. Antibodies: IFI16 9641, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 4 Immunofluorescence Validation of IFI16 in Human Molt4 Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed Molt4 cells labeling IFI16 with 9641 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 5 Immunohistochemistry Validation of IFI16 in Human Testis</strong><br> Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-IFI16 antibody (9641) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 6 Immunohistochemistry Validation of IFI16 in Mouse Liver</strong><br> Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-IFI16 antibody (9641) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 7 Immunohistochemistry Validation of IFI16 in Rat Testis</strong><br> Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-IFI16 antibody (9641) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 1 WB Validation in Human and Rat Cell Lines </strong><br> Loading: 15 μg of lysate per lane. Antibodies: IFI16 9641, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 2 Western Blot Validation in Mouse Tissues </strong><br> Loading: 15 μg of lysates per lane. Antibodies: IFI16 9641, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 3 Western Blot Validation in Rat Testis</strong><br> Loading: 15 μg of lysate per lane. Antibodies: IFI16 9641, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 4 Immunofluorescence Validation of IFI16 in Human Molt4 Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed Molt4 cells labeling IFI16 with 9641 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 5 Immunohistochemistry Validation of IFI16 in Human Testis</strong><br> Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-IFI16 antibody (9641) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6 Immunohistochemistry Validation of IFI16 in Mouse Liver</strong><br> Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-IFI16 antibody (9641) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7 Immunohistochemistry Validation of IFI16 in Rat Testis</strong><br> Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-IFI16 antibody (9641) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Documents

Further Information

Additional Names:
IFI16 Antibody: Gamma-interferon-inducible protein 16, Ifi-16, Interferon-inducible myeloid differentiation transcriptional activator, IFNGIP1
Application Note:
WB: 2 μg/mL; IF: 20 μg/mL; IHC: 1 μg/mL.

Antibody validated: Western Blot in human, mouse, and rat samples; Immunofluorescence in human samples; Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.
Background:
IFI16 Antibody: IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer. Increased expression of IFI16 predicts adverse prognosis in multiple myeloma. IFI16 isoforms with cytoplasmic and nuclear locations play differential roles in recognizing invaded DNA viruses. IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection. Nuclear sensor IFI16 inhibits the function of Hepatitis B virus covalently closed circular DNA by integrating innate immune activation and epigenetic suppression.
Background References:
  • Ka et al. Cell Rep. 2021; 37(12):110138
  • Huang et al. Pharmacogenomics J. 2021; 21(4):520-532
  • Li et al. J Immunol. 2021; 207(11):2699-2709
  • Jiang et al. Nat Microbiol. 2021; 6(7):932-945
  • Yang et al. Hepatology 2020; 71(4):1154-1169
Buffer:
IFI16 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
Homology:
Predicted species reactivity based on immunogen sequence: Gorilla (100%), chimpanzee (100%)
Immunogen:
Anti-IFI16 antibody (9641) was raised against a peptide corresponding to 16 amino acids near the carboxyl terminus of human IFI16.

The immunogen is located within the last 50 amino acids of IFI16.
ISOFORMS:
Human IFI16 has 4 isoforms, including isoform 1 (785aa, 8.3kD), isoform 2 (729aa, 82.1kD), isoform 3(673aa, 75.9kD), and isoform 4 (729aa, 82.6kD)
NCBI Gene ID #:
3428
NCBI Official Name:
interferon gamma inducible protein 16
NCBI Official Symbol:
IFI16
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 88kD

Observed: 88 kD
Protein Accession #:
Q16666.3
Protein GI Number:
118572657
Purification:
IFI16 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis, Cancer, Cell Cycle, Transcription
Swissprot #:
Q16666
User NOte:
Optimal dilutions for each application to be determined by the researcher.