EGFR Antibody [528], Rabbit IgG

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ProSci
Product Code: PSI-24-138
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-138-0.2mg0.2mg£681.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: 528
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Immunoprecipitation (IP)
  • Neutralisation
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluoresence staining of fixed HeLa cells with anti-EGFR antibody 528 (</b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton and stained with the chimeric r version of t 10ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-138- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor®?488 secondary antibody.
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<b> Immunofluoresence staining of fixed HeLa cells with anti-EGFR antibody 528 (</b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton and stained with the chimeric r version of t 10ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-138- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor®?488 secondary antibody.

Documents

Further Information

Additional Names:
ERBB, ERBB1, HER1, PIG61, mENA, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, proto-oncogene c-ErbB-1, receptor tyrosine-protein kinase erbB-1, epidermal growth f
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Partially purified EGFR from A431 cells.
NCBI Gene ID #:
718
NCBI Official Name:
complement C3
NCBI Official Symbol:
C3
NCBI Organism:
Homo sapiens
Research Area:
Cancer, Signal Transduction
Swissprot #:
P00533
User NOte:
For research use only.