IL-5 Antibody [2E3], Rabbit IgG

ProSci
Product Code: PSI-24-640
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-640-0.2mg0.2mg£681.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: 2E3
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Neutralisation
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Flow-cytometry using the Anti-IL-5 antibody 2E3 ( </b> Human peripheral blood leukocytes were rested for 4 hours. The cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r version of 2E3 (24-640- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.

<b> Flow-cytometry using the Anti-IL-5 antibody 2E3 ( </b> Human peripheral blood leukocytes were rested for 4 hours. The cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r version of 2E3 (24-640- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.

Further Information

Additional Names:
interleukin 5, IL5, IL 5, Eosinophil differentiation factor
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was raised by immunising CAF1 mice with human recombinant IL-5.
Research Area:
Chemokines & Cytokines, Immunology
Swissprot #:
C9JQP9
User NOte:
For research use only.