CD25 Antibody [2E4], Rabbit IgG

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ProSci
Product Code: PSI-31-544
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-31-544-0.2mg0.2mg£681.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: 2E4
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Depletion
  • Flow Cytometry
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Flow cytometry using the Anti-CD25 antibody 2E4 ( </b> Paraformaldehyde fixed mouse splenocytes permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of 2E4 (31-544- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Flow cytometry using the Anti-CD25 antibody 2E4 ( </b> Paraformaldehyde fixed mouse splenocytes permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of 2E4 (31-544- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Documents

Further Information

Additional Names:
IL-2-RA, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2R subunit alpha, IL2-RA, p55
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
The original version of this antibody was raised to recognize mouse CD25.
NCBI Gene ID #:
16184
NCBI Official Name:
interleukin 2 receptor, alpha chain
NCBI Official Symbol:
Il2ra
NCBI Organism:
Mus musculus
Research Area:
Immunology, Other, Cancer, Chemokines & Cytokines
Swissprot #:
P01590
User NOte:
For research use only.