DC-SIGNR Antibody [16E7], Rabbit IgG

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ProSci
Product Code: PSI-24-134
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-134-0.2mg0.2mg£681.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: 16E7
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Immunohistochemistry (IHC)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Western Blot using anti-DC-SIGNR (CLEC4M) antibody 16E7 (</b> Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of 16E7 (24-134) at 0.1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for DC-SIGNR is 45.3kDa- though 9 other isoforms of this protein are known ranging in size from 22.4-44.7kDa (Uniprot ID: Q9H2X3). DC-SIGNR is also glycosylated at several positions. 24-134 successfully detected the canonical human DC-SIGNR- as well as multiple other isoforms.
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<b> Western Blot using anti-DC-SIGNR (CLEC4M) antibody 16E7 (</b> Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of 16E7 (24-134) at 0.1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for DC-SIGNR is 45.3kDa- though 9 other isoforms of this protein are known ranging in size from 22.4-44.7kDa (Uniprot ID: Q9H2X3). DC-SIGNR is also glycosylated at several positions. 24-134 successfully detected the canonical human DC-SIGNR- as well as multiple other isoforms.

Documents

Further Information

Additional Names:
CD299, C-type lectin domain family 4 member M, CD209 antigen-like protein 1, DC-SIGN-related protein, CLEC4M, Dendritic cell-specific ICAM-3-grabbing non-integrin 2, DC-SIGN2, Liver/lymph node-specific ICAM-3-grabbing non-integrin, L-SIGN
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
CD299
NCBI Gene ID #:
10332
NCBI Official Name:
HGNC:HGNC:13523
NCBI Official Symbol:
CLEC4M
NCBI Organism:
Homo sapiens
Research Area:
Immunology
Swissprot #:
Q9H2X3
User NOte:
For research use only.