CD16/CD32 Antibody [2.4G2], Rat IgG2b

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ProSci
Product Code: PSI-24-122
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-122-0.2mg0.2mg£681.00
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Overview

Antibody Isotype: IgG2b kappa
Antibody Clonality: Monoclonal
Antibody Clone: 2.4G2
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Blocking
  • Flow Cytometry
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b>Blocking of Fc-receptors by anti-CD16/32 antibody.</b> <i>Ex vivo</i> murine (C57BL6/J) monocytes and neutrophils (from zymosan-elicited peritoneal exudate- left and middle panel) as well as bone marrow-derived macrophages (BMDMs- right panel) were stained with a murine anti-human antibody (fluorescently lAF647) human-specific anti-CD16) with or without pre-incubation with anti-mouse CD16/CD32 (Fc-receptors) antibody (24-122- clone 2.4G2). Pre-incubation of cells with anti-CD16/CD32 reduced non-specific binding of the fluorescently lntibody to all cell types analysed and shows that this anti-CD16/CD32 antibody acts as an Fc-blocking reagent.
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<b>Blocking of Fc-receptors by anti-CD16/32 antibody.</b> <i>Ex vivo</i> murine (C57BL6/J) monocytes and neutrophils (from zymosan-elicited peritoneal exudate- left and middle panel) as well as bone marrow-derived macrophages (BMDMs- right panel) were stained with a murine anti-human antibody (fluorescently lAF647) human-specific anti-CD16) with or without pre-incubation with anti-mouse CD16/CD32 (Fc-receptors) antibody (24-122- clone 2.4G2). Pre-incubation of cells with anti-CD16/CD32 reduced non-specific binding of the fluorescently lntibody to all cell types analysed and shows that this anti-CD16/CD32 antibody acts as an Fc-blocking reagent.

Documents

Further Information

Additional Names:
Fc? III/II Receptor, Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III, Fc-gamma RIII, FcRIII, CD16, Low affinity immunoglobulin gamma Fc region receptor II, Fc gamma receptor IIB, Fc-gamma RII, Fc-gamma-RIIB, FcRII IgG Fc rec
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Mouse BALB/c Macrophage J774 Cell Line.
NCBI Gene ID #:
14130
NCBI Official Name:
Fc receptor, IgG, low affinity Iib
NCBI Official Symbol:
Fcgr2b
NCBI Organism:
Mus musculus
Research Area:
Other, Immunology
Swissprot #:
P08508; P08101
User NOte:
For research use only.