Haemoglobin Antibody [2A1], Rabbit IgG

ProSci
Product Code: PSI-24-570
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-570-0.2mg0.2mg£681.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: 2A1
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

1 / 1
<b> Immunofluorescence staining of fixed mouse splenocytes with anti-Haemoglobin antibody 2A1 (</b> Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips- permewith 0.15% Triton stained with the chimeric r version of 2A1 (24-570) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-570- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.

<b> Immunofluorescence staining of fixed mouse splenocytes with anti-Haemoglobin antibody 2A1 (</b> Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips- permewith 0.15% Triton stained with the chimeric r version of 2A1 (24-570) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-570- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.

Further Information

Additional Names:
Hemoglobin, Hb
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Murine hybridomas were generated using spleen cells from aging (8?10 month old) NZB/W F1 animals. Hybridomas secreting antibodies reactive to murine hemoglobin were subcloned by limiting dilution and antibody isotypes determined using commercial reagents. Eight-week-old NZB/W F1 were immunized subcutaneously with 50 mg mHb antigen emulsified in Complete Fruend?s Adjuvant.
Research Area:
Immunology
Swissprot #:
P01942
User NOte:
For research use only.