RAGE Antibody [2A11], Rabbit IgG

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ProSci
Product Code: PSI-24-660
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-660-0.2mg0.2mg£681.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: 2A11
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Surface Plasma Resonance
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Flow-cytometry using the Anti-RAGE antibody 2A11 ( </b> A549 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- shaded line) or the r version of 2A11 (24-660- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Western Blot using anti-RAGE antibody 2A11 ( </b> Human lung lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric r version of 2A11 (24-660) at 0.03 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
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<b> Flow-cytometry using the Anti-RAGE antibody 2A11 ( </b> A549 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- shaded line) or the r version of 2A11 (24-660- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
<b> Western Blot using anti-RAGE antibody 2A11 ( </b> Human lung lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric r version of 2A11 (24-660) at 0.03 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Documents

Further Information

Additional Names:
AGER, advanced glycosylation end product-specific receptor, receptor for advanced glycosylation end products
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Human RAGE protein.
NCBI Gene ID #:
177
NCBI Official Name:
advanced glycosylation end-product specific receptor
NCBI Official Symbol:
AGER
NCBI Organism:
Homo sapiens
Research Area:
Other
Swissprot #:
Q15109?
User NOte:
For research use only.