C5aR Antibody [32-G1], Rabbit IgG

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ProSci
Product Code: PSI-24-780
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-780-0.2mg0.2mg£681.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: 32-G1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluorescence staining of fixed U937 cells with anti-C5aR antibody 32-G1 (</b> Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips stained with the chimeric r version 32-G1 (24-780) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-780- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.
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<b> Immunofluorescence staining of fixed U937 cells with anti-C5aR antibody 32-G1 (</b> Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips stained with the chimeric r version 32-G1 (24-780) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-780- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.

Documents

Further Information

Additional Names:
CD88, C5a anaphylatoxin chemotactic receptor, C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, pC5aR, 32G-1
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was raised by immunizing mice a synthetic C5aR C-terminal phosphopeptide that contained phosphoserine instead of serine at position 334 and also at the neighboring phosphorylation sites Ser-332 and Ser-338.
NCBI Gene ID #:
728
NCBI Official Name:
complement C5a receptor 1
NCBI Official Symbol:
C5AR1
NCBI Organism:
Homo sapiens
Research Area:
Other, Immunology
Swissprot #:
P21730
User NOte:
For research use only.