Lymphotoxin alpha Antibody [3F12.2D3], Rabbit IgG

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ProSci
Product Code: PSI-24-516
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-516-0.2mg0.2mg£681.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: 3F12.2D3
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Neutralisation
  • Surface Plasma Resonance
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Western Blot using anti-Lymphotoxin alpha antibody 3F12.2D3 (</b> Lipopolysaccharide(LPS)-treated human peripheral blood monocyte (PBM) lysate (35ug protein in RIPA buffer) was resolved on a 15% SDS PAGE gel and blots probed with the r-chimeric version of 3F12.2D3 (24-516) at 2 ug/ml before detection using an anti-rondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for Lymphotoxin alpha is 22.3kDa- though the form shown here is likely glycosylated. 24-516 successfully detected Lymphotoxin alpha in LPS-treated human PBM samples.
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<b> Western Blot using anti-Lymphotoxin alpha antibody 3F12.2D3 (</b> Lipopolysaccharide(LPS)-treated human peripheral blood monocyte (PBM) lysate (35ug protein in RIPA buffer) was resolved on a 15% SDS PAGE gel and blots probed with the r-chimeric version of 3F12.2D3 (24-516) at 2 ug/ml before detection using an anti-rondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for Lymphotoxin alpha is 22.3kDa- though the form shown here is likely glycosylated. 24-516 successfully detected Lymphotoxin alpha in LPS-treated human PBM samples.

Documents

Further Information

Additional Names:
LT?3 chimeric 3F12,LT,, LT?, Tumor necrosis factor beta, Tumor necrosis factor ?, Tumor necrosis factor-beta, tumor necrosis factor-?, TNF beta, TNF-beta, TNF ?, TNF-?, Lymphotoxin-alpha, Lymphotoxin alpha
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTα expressed in E. coli - the hybridoma clone was selected for its ability to bind LTα3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.
NCBI Gene ID #:
4049
NCBI Official Name:
lymphotoxin alpha
NCBI Official Symbol:
LTA
NCBI Organism:
Homo sapiens
Research Area:
Immunology, Other
Swissprot #:
P01374
User NOte:
For research use only.