erbB-2 (Her-2/neu) Antibody [4D5-8 (trastuzumab)], Rabbit IgG

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ProSci
Product Code: PSI-24-088
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-088-0.2mg0.2mg£681.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: 4D5-8 (trastuzumab)
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunohistochemistry (IHC)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunohistochemical staining of paraffin embedded human breast tissue using anti-erbB-2 antibody (4D5-8 (trastuzumAnti-erbB-2 (Her-2/neu) staining of paraffin embedded human breast tissue using the rmeric version of 4D5-8 (trastuzum88). Antigen retreival was achieved by microwaving in citrate buffer (pH6)- followed by blocking with protein block serum-free buffer (Dako- cat. #X0909). Primary antibody incubation with 24-088 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-r HRP secondary antibody (Dako cat#K4009) for 20 mins followed by Ddiaminobenzidine)- and counter-staining with haemotoxylin. Weak membrane staining exclusively of ductal epithelial cells may be observed. Recommended concentration- 4-6 ug/ml.
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<b> Immunohistochemical staining of paraffin embedded human breast tissue using anti-erbB-2 antibody (4D5-8 (trastuzumAnti-erbB-2 (Her-2/neu) staining of paraffin embedded human breast tissue using the rmeric version of 4D5-8 (trastuzum88). Antigen retreival was achieved by microwaving in citrate buffer (pH6)- followed by blocking with protein block serum-free buffer (Dako- cat. #X0909). Primary antibody incubation with 24-088 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-r HRP secondary antibody (Dako cat#K4009) for 20 mins followed by Ddiaminobenzidine)- and counter-staining with haemotoxylin. Weak membrane staining exclusively of ductal epithelial cells may be observed. Recommended concentration- 4-6 ug/ml.

Documents

Further Information

Additional Names:
CD340, Metastatic lymph node gene 19 protein (MLN 19), Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, p185HER2, EGFR
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
A431 cells (human epidermoid carcinoma) (over)expressing EGFR.
NCBI Gene ID #:
2064
NCBI Official Name:
erb-b2 receptor tyrosine kinase 2
NCBI Official Symbol:
ERBB2
NCBI Organism:
Homo sapiens
Research Area:
Cancer, Other
Swissprot #:
P04626
User NOte:
For research use only.