CD40 Antibody [5D12], Rabbit IgG

ProSci
Product Code: PSI-24-515
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-515-0.2mg0.2mg£681.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: 5D12
Regulatory Status: RUO
Target Species:
  • Cynomolgus Monkey
  • Human
  • Marmoset
Applications:
  • Blocking
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Surface Plasma Resonance
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluorescence staining of fixed Daudi cells with anti-CD40 antibody 5D12 (</b> Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips- permewith 0.15% Triton and stained with the chimeric r version of 5D12 (24-515) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-515- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor®?488 secondary antibody.

<b> Immunofluorescence staining of fixed Daudi cells with anti-CD40 antibody 5D12 (</b> Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips- permewith 0.15% Triton and stained with the chimeric r version of 5D12 (24-515) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-515- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor®?488 secondary antibody.

Further Information

Additional Names:
TNFRS5,Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Anti-CD40 mAb 5D12, was generated by immunizing mice with sf9 insect cells expressing recombinant human CD40 and selected for the ability to bind EBV-immortalized human B cells. The?variable regions from mu5D12 were cloned and used to construct chimeric humanized IgG4?5D12.
NCBI Gene ID #:
958
NCBI Official Name:
CD40 molecule
NCBI Official Symbol:
CD40
NCBI Organism:
Homo sapiens
Research Area:
Immunology, Other, Neuroscience
Swissprot #:
P25942
User NOte:
For research use only.