Tim-4 Antibody [5G3], Rabbit IgG

ProSci
Product Code: PSI-24-617
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-617-0.2mg0.2mg£681.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: 5G3
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Blocking
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluorescence staining of fixed mouse splenocytes with anti-Tim-4 antibody 5G3 (</b> Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes permewith 0.15% Triton and stained with the chimeric mouse IgG1 version of 5G3 (24-617) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-617- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody (0) followed by Alexa Fluor®?488 secondary antibody.

<b> Immunofluorescence staining of fixed mouse splenocytes with anti-Tim-4 antibody 5G3 (</b> Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes permewith 0.15% Triton and stained with the chimeric mouse IgG1 version of 5G3 (24-617) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-617- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody (0) followed by Alexa Fluor®?488 secondary antibody.

Further Information

Additional Names:
TIMD4, SMUCKLER, SMUCKLER, TIM4, Spleen, mucin-containing, knockout of lymphotoxin protein, T-cell immunoglobulin and mucin domain containing 4, T-cell immunoglobulin and mucin domain-containing protein 4, T-cell immunoglobulin mucin receptor 4, T-cell membrane protein 4, TIM-4, TIMD-4
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was raised by immunising female Lewis rats (Harlan Sprague-Dawley) with Tim-4:Ig fusion protein in complete Freund's adjuvant.
NCBI Gene ID #:
276891
NCBI Official Name:
Timd4
NCBI Official Symbol:
Timd4
NCBI Organism:
Mus musculus
Research Area:
Immunology, Neuroscience, Cell Cycle, Cancer
Swissprot #:
Q6U7R4
User NOte:
For research use only.