CD43 Antibody [84-3C1], Rabbit IgG

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ProSci
Product Code: PSI-24-755
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-755-0.2mg0.2mg£681.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: 84-3C1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluorescence staining of fixed K562 cells with anti-CD43 antibody 84-3C1 (</b> Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix™ coverslips stained with the chimeric r version of 84-3C1 (24-755) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-755- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
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<b> Flow cytometry using the anti-CD43 antibody 84-3C1 ( </b> K562 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of 84-3C1 (24-755- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Immunofluorescence staining of fixed K562 cells with anti-CD43 antibody 84-3C1 (</b> Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix™ coverslips stained with the chimeric r version of 84-3C1 (24-755) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-755- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
<b> Flow cytometry using the anti-CD43 antibody 84-3C1 ( </b> K562 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of 84-3C1 (24-755- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Documents

Further Information

Additional Names:
Leukosialin, Sialophorin, GPL115, Galactoglycoprotein, GALGP, Leukocyte sialoglycoprotein
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was raised by immunizng mice with stimulated human leukocytes.
NCBI Gene ID #:
6693
NCBI Official Name:
sialophorin
NCBI Official Symbol:
SPN
NCBI Organism:
Homo sapiens
Research Area:
Cancer, Immunology, Other
Swissprot #:
P16150
User NOte:
For research use only.