IL-17F Antibody [8F5.1A9], Rabbit IgG

Contact us
ProSci
Product Code: PSI-24-678
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-678-0.2mg0.2mg£681.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: 8F5.1A9
Regulatory Status: RUO
Target Species: Mouse
Application: Flow Cytometry
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

1 / 1
<b> Flow-cytometry using the Anti-IL-17F antibody 8F5.1A9 ( </b> Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r version of 8F5.1A9 (24-678- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.
❮❯

<b> Flow-cytometry using the Anti-IL-17F antibody 8F5.1A9 ( </b> Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r version of 8F5.1A9 (24-678- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.

Documents

Further Information

Additional Names:
Interleukin-17F, IL17F
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was raised by immunising mice with murine IL-17F coupled to ovalbumin to generate a highly immunogenic complex.
NCBI Gene ID #:
257630
NCBI Official Name:
interleukin 17F
NCBI Official Symbol:
Il17f
NCBI Organism:
Mus musculus
Research Area:
Chemokines & Cytokines
Swissprot #:
Q7TNI7
User NOte:
For research use only.