Beta-2 microglobulin Antibody [BBM.1], Rabbit IgG

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ProSci
Product Code: PSI-24-233
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-233-0.2mg0.2mg£681.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: BBM.1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Depletion
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluoresence staining of fixed HeLa cells with anti-Beta-2 microglobulin antibody BBM.1 (</b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of BBM.1 (24-233) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-233- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor®?488 secondary antibody.
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<b> Western Blot using anti-Beta-2 microglobulin antibody BBM.1 (</b> HeLa cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of BBM.1 (24-233) at 0.03 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-2 microglobulin is 13.7 kDa. 24-233 successfully detected human Beta-2 microglobulin in both HeLa cell extract and human duodenum samples.
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<b> Immunofluoresence staining of fixed HeLa cells with anti-Beta-2 microglobulin antibody BBM.1 (</b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of BBM.1 (24-233) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-233- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor®?488 secondary antibody.
<b> Western Blot using anti-Beta-2 microglobulin antibody BBM.1 (</b> HeLa cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of BBM.1 (24-233) at 0.03 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-2 microglobulin is 13.7 kDa. 24-233 successfully detected human Beta-2 microglobulin in both HeLa cell extract and human duodenum samples.

Documents

Further Information

Additional Names:
B2M, Beta-2-microglobulin, CDABP0092, HDCMA22P, BBM1
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Molt 4, a human T cell line.
NCBI Gene ID #:
1913
NCBI Official Name:
ectrodactyly, ectodermal dysplasia and cleft lip/palate syndrome 1
NCBI Official Symbol:
EEC1
NCBI Organism:
Homo sapiens
Research Area:
Cancer, Immunology, Other
Swissprot #:
P61769
User NOte:
For research use only.