CD131 Antibody [BION-1], Rabbit IgG

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ProSci
Product Code: PSI-24-572
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-572-0.2mg0.2mg£681.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: BION-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Blocking
  • Crystallography
  • Functional Study
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Flow-cytometry using the Anti-CD131 antibody BION-1 ( </b> U937 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r version of BION-1 ((24-572- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Flow-cytometry using the Anti-CD131 antibody BION-1 ( </b> U937 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r version of BION-1 ((24-572- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Documents

Further Information

Additional Names:
CSF2RB, CD131, IL3RB, IL-3R common beta subunit, IL5RB, colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage), SMDP5, Common Beta-Chain, CDw131, cytokine receptor common subunit beta, beta-c, CD131, Beta Common Chain
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
BALB/c mice were immunized intraperitonally with COS cells transfected DQP (Domain 4 of the common beta-chain) expression constructs. The immunizations were repeated 4 times at 2-week intervals. Four weeks after the final immunization, a mouse was boosted with COS transfectants intravenously. Three days later, splenocytes were harvested and fused with NS-1 myeloma cells. Hybridoma supernatants were screened on CHO DQP cells by flow cytometry, with untransfected CHO cells as a control. All antibodies were from single hybridoma clones as selected by a limiting dilution method. MoAbs were purified from ascites fluid or hybridoma supernatant by a protein A sepharose column.
NCBI Gene ID #:
1439
NCBI Official Name:
colony stimulating factor 2 receptor subunit beta
NCBI Official Symbol:
CSF2RB
NCBI Organism:
Homo sapiens
Research Area:
Immunology
Swissprot #:
P32927
User NOte:
For research use only.