GCN4 Antibody [C11L34], Mouse IgG1

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ProSci
Product Code: PSI-24-378
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-378-0.2mg0.2mg£681.00
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Overview

Host Type: Mouse
Antibody Isotype: IgG1 lambda
Antibody Clonality: Monoclonal
Antibody Clone: C11L34
Regulatory Status: RUO
Target Species: Saccharomyces cerevisiae
Applications:
  • Immunoprecipitation (IP)
  • Surface Plasma Resonance
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluorescence staining of fixed HeLa cells expressing mCherry tagged GCN4 with anti-GCN4 antibody C11L34 (</b> Immunofluorescence analysis of fixed HeLa cells stained with the chimeric mouse IgG1 version of C11L34 (24-378) followed by an Alexa Fluor®?488 secondary antibody- showing mitochondrial staining. Colocalisation with mCherry tagged GCN4 demonstrates the very low off target/non-specific binding of this antibody. The nuclear and mitochondiral DNA stain is DAPI (blue). Panels show; a) mCherry tagged- mitochondrially targetted GCN4- b) 24-378 with Alexa Fluor®?488 secondary antibody- c) DNA stained with DAPI- d) Merged channels. Images courtesy of Luming Chen (Kim Orth luthwestern Medical Center).
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<b> Immunofluorescence staining of fixed HeLa cells expressing mCherry tagged GCN4 with anti-GCN4 antibody C11L34 (</b> Immunofluorescence analysis of fixed HeLa cells stained with the chimeric mouse IgG1 version of C11L34 (24-378) followed by an Alexa Fluor®?488 secondary antibody- showing mitochondrial staining. Colocalisation with mCherry tagged GCN4 demonstrates the very low off target/non-specific binding of this antibody. The nuclear and mitochondiral DNA stain is DAPI (blue). Panels show; a) mCherry tagged- mitochondrially targetted GCN4- b) 24-378 with Alexa Fluor®?488 secondary antibody- c) DNA stained with DAPI- d) Merged channels. Images courtesy of Luming Chen (Kim Orth luthwestern Medical Center).

Documents

Further Information

Additional Names:
General Control Protein 4
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This full-length, reformatted mouse antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Biotinylated GCN4-peptide (HLENEVARLKK).
NCBI Gene ID #:
856709
NCBI Official Name:
amino acid starvation-responsive transcription factor GCN4
NCBI Official Symbol:
GCN4
NCBI Organism:
Saccharomyces cerevisiae S288C
Research Area:
Other, Cell Biology
Swissprot #:
P03069
User NOte:
For research use only.