HLA class II Antibody [F3.3], Rabbit IgG

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ProSci
Product Code: PSI-24-238
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-238-0.2mg0.2mg£681.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: F3.3
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Western Blot using anti-HLA class II histocompatibility antigen DR antibody F3.3 (</b> Human spleen (A)- human tonsil (B) and Daudi cell lysate (C) samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of F3.3 (24-238) at 0.01 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for HLA II DR is ~30 kDa- depending upon the exact DR isoform expressed (c.f. Klitz et al- PMID: 12974796). 24-238 successfully detected human HLA class II histocompatibility antigen DR in human spleen and tonsil lysate as well as Daudi cell lysate.
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<b> Flow-cytometry using anti-HLA class II antibody F3.3 (</b> Human leukocytes were stained with an isotype control (3.0- panel A) or the rmeric version of F3.3 (24-238- panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing- bound antibody was detected using a AF488 conjugated donkey anti-ribody ( and cells analysed on a FACSCanto flow-cytometer.
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<b> Western Blot using anti-HLA class II histocompatibility antigen DR antibody F3.3 (</b> Human spleen (A)- human tonsil (B) and Daudi cell lysate (C) samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of F3.3 (24-238) at 0.01 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for HLA II DR is ~30 kDa- depending upon the exact DR isoform expressed (c.f. Klitz et al- PMID: 12974796). 24-238 successfully detected human HLA class II histocompatibility antigen DR in human spleen and tonsil lysate as well as Daudi cell lysate.
<b> Flow-cytometry using anti-HLA class II antibody F3.3 (</b> Human leukocytes were stained with an isotype control (3.0- panel A) or the rmeric version of F3.3 (24-238- panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing- bound antibody was detected using a AF488 conjugated donkey anti-ribody ( and cells analysed on a FACSCanto flow-cytometer.

Documents

Further Information

Additional Names:
HLA-DR, HLA class II related epitope
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Research Area:
Immunology, Infectious Disease
User NOte:
For research use only.