ED-B domain of fibronectin Antibody [G4], Rabbit IgG

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ProSci
Product Code: PSI-24-457
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-457-0.2mg0.2mg£681.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: G4
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluoresence staining of fixed HeLa cells with anti-ED-B domain of fibronectin antibody G4 (</b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of G4 (24-457) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-457- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor®?488 secondary antibody.
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<b> Immunofluoresence staining of fixed HeLa cells with anti-ED-B domain of fibronectin antibody G4 (</b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of G4 (24-457) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-457- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor®?488 secondary antibody.

Documents

Further Information

Additional Names:
EDB(+) FN, Extra domain-B containing fibronectin
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
2D-PAGE gel isolated human Extra-Domain containing fibronectin from lysate of human melanoma COLO-38 cells.
NCBI Gene ID #:
2335
NCBI Official Name:
fibronectin 1
NCBI Official Symbol:
FN1
NCBI Organism:
Homo sapiens
Research Area:
Other, Signal Transduction, Immunology, Cell Biology, Cancer
Swissprot #:
P02751
User NOte:
For research use only.