CD64 Antibody [H22], Rabbit IgG

ProSci
Product Code: PSI-24-496
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-496-0.2mg0.2mg£681.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: H22
Regulatory Status: RUO
Target Species: Human
Applications:
  • Competition Study
  • Cytotoxicity
  • Flow Cytometry
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluorescence staining of fixed U937 cells with anti-CD64 antibody H22 (</b> Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips- permewith 0.15% Triton and stained with the chimeric r version of H22 (24-496) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-496- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor®?488 secondary antibody.

<b> Immunofluorescence staining of fixed U937 cells with anti-CD64 antibody H22 (</b> Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips- permewith 0.15% Triton and stained with the chimeric r version of H22 (24-496) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-496- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor®?488 secondary antibody.

Further Information

Additional Names:
Fc?RI, High affinity immunoglobulin gamma Fc receptor I, IgG Fc receptor I, Fc-gamma RI, FcRI, Fc-gamma RIA
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This recombinant antibody was prepared by cloning the single chain fragment (scFv) derived from the murine anti-human CD64 mAb m22 into the bacterial expression vector pBM1.1, and fusing it to a deletion mutant of Pseudomonas exotoxin A (ETA'??).
NCBI Gene ID #:
2209
NCBI Official Name:
Fc gamma receptor Ia
NCBI Official Symbol:
FCGR1A
NCBI Organism:
Homo sapiens
Research Area:
Cancer, Immunology, Chemokines & Cytokines
Swissprot #:
P12314
User NOte:
For research use only.