EpCAM Antibody [HEA125], Mouse IgG1

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ProSci
Product Code: PSI-24-715
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-715-0.2mg0.2mg£681.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: HEA125
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Radioimmunoassay (RIA)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Western Blot using anti-EpCAM antibody HEA125 ( </b> human breast carcinoma(A) and human colorectal carcinoma(B) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of HEA125 (24-715) at 1 ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
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<b> Immunofluorescence staining of Caco-2 cells using anti-EpCAM (HEA125 </b> Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells stained with the chimeric mouse IgG version of HEA125 (24-715) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom .1- DAPI- merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (.1) followed by Alexa Fluor® 488 secondary antibody.
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<b> Flow cytometry using the Anti-EpCAM antibody HEA125 ( </b> Caco-2 cells were stained with anti-unknown specificity antibody (.1; isotype control- black line) or the mouse IgG1 version of HEA125 (24-715- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Western Blot using anti-EpCAM antibody HEA125 ( </b> human breast carcinoma(A) and human colorectal carcinoma(B) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of HEA125 (24-715) at 1 ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
<b> Immunofluorescence staining of Caco-2 cells using anti-EpCAM (HEA125 </b> Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells stained with the chimeric mouse IgG version of HEA125 (24-715) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom .1- DAPI- merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (.1) followed by Alexa Fluor® 488 secondary antibody.
<b> Flow cytometry using the Anti-EpCAM antibody HEA125 ( </b> Caco-2 cells were stained with anti-unknown specificity antibody (.1; isotype control- black line) or the mouse IgG1 version of HEA125 (24-715- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Documents

Further Information

Additional Names:
CD326, Ep-CAM, Epithelial cell adhesion molecule, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, EGP, Epithelial glycoprotein 314, EGP314, hEGP314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, HEA 125
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was raised by immunising BALB/c mice with the human colon carcinoma cell line HT-29.
NCBI Gene ID #:
4072
NCBI Official Name:
epithelial cell adhesion molecule
NCBI Official Symbol:
EPCAM
NCBI Organism:
Homo sapiens
Research Area:
Cancer, Other
Swissprot #:
P16422
User NOte:
For research use only.