MUC1 Antibody [HMFG2], Rabbit IgG

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ProSci
Product Code: PSI-24-479
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-479-0.2mg0.2mg£681.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG lambda
Antibody Clonality: Monoclonal
Antibody Clone: HMFG2
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Imaging
  • Immunohistochemistry (IHC)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Flow-cytometry using the anti-MUC1 antibody HMFG2 (</b> MCF-7 cells were stained with unimmunized r antibody (black line) or the rmeric version of HMFG2 (24-479- blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using an anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.
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<b> Immunohistochemical staining of human lung tissue using anti-MUC1 antibody (HMFG2 </b> Anti-MUC1 (Mucin-1) staining of paraffin embedded human lung tissue using the rmeric version of HMFG2 (24-479). Antigen retreival was acheived by microwaving in citrate buffer (pH6)- followed by blocking with protein block serum-free buffer (Dako- cat. #X0909). Primary antibody incubation with 24-479 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-r HRP secondary antibody (Dako cat#K4009) for 20 mins followed by Ddiaminobenzidine)- and counter-staining with haemotoxylin. Strong staining of type II pneumocytes may be observed. Recommended concentration- 1-2 ug/ml.
4 / 4
<b> Immunofluorescence staining of fixed MCF-7 cells with anti-MUC1 antibody HMFG2 (</b> Immunofluorescence analysis of unpermearaformaldehyde fixed MCF-7 cells on Shi-fix™ coverslips stained with the chimeric rsion of HMFG2 (24-479) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-479- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor®?488 secondary antibody.
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<b> Flow-cytometry using the anti-MUC1 antibody HMFG2 (</b> MCF-7 cells were stained with unimmunized r antibody (black line) or the rmeric version of HMFG2 (24-479- blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using an anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.
<b> Immunohistochemical staining of human lung tissue using anti-MUC1 antibody (HMFG2 </b> Anti-MUC1 (Mucin-1) staining of paraffin embedded human lung tissue using the rmeric version of HMFG2 (24-479). Antigen retreival was acheived by microwaving in citrate buffer (pH6)- followed by blocking with protein block serum-free buffer (Dako- cat. #X0909). Primary antibody incubation with 24-479 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-r HRP secondary antibody (Dako cat#K4009) for 20 mins followed by Ddiaminobenzidine)- and counter-staining with haemotoxylin. Strong staining of type II pneumocytes may be observed. Recommended concentration- 1-2 ug/ml.
<b> Immunofluorescence staining of fixed MCF-7 cells with anti-MUC1 antibody HMFG2 (</b> Immunofluorescence analysis of unpermearaformaldehyde fixed MCF-7 cells on Shi-fix™ coverslips stained with the chimeric rsion of HMFG2 (24-479) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-479- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor®?488 secondary antibody.

Documents

Further Information

Additional Names:
Muc-1
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
High-MW glycoprotein of the human milk fat globule.
NCBI Gene ID #:
4582
NCBI Official Name:
HGNC:HGNC:7508
NCBI Official Symbol:
MUC1
NCBI Organism:
Homo sapiens
Research Area:
Cancer, Other
Swissprot #:
P15941
User NOte:
For research use only.