H4 K8Ac; K12Ac; K16Ac Antibody [KM-2], Rabbit IgG

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ProSci
Product Code: PSI-24-811
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-811-0.2mg0.2mg£681.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: KM-2
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Flow cytometry using the anti-H4 K8Ac; K12Ac; K16Ac antibody KM-2 ( </b> HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of KM-2 (24-811- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Flow cytometry using the anti-H4 K8Ac; K12Ac; K16Ac antibody KM-2 ( </b> HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of KM-2 (24-811- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Documents

Further Information

Additional Names:
histone 4, nucleosome, H4K8Ac, H4K12Ac, H4K16Ac, acetyl-Histone H4, Histone H4 acetylated at lysine, KM2
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
KM-2 antibody was derived from an MRL/lpr lupus mouse.
Research Area:
Other, Cell Cycle, Apoptosis, Immunology
User NOte:
For research use only.